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Glycobiology Advance Access originally published online on August 6, 2008
Glycobiology 2008 18(11):871-881; doi:10.1093/glycob/cwn074
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

The structure and proinflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs

Teresa Ieranò2, Alba Silipo2, Luisa Sturiale3, Domenico Garozzo3, Heather Brookes4, C M Anjam Khan5, Clare Bryant4, F Kate Gould6,7, Paul A Corris7,8, Rosa Lanzetta2, Michelangelo Parrilli2, Anthony De Soyza8 and Antonio Molinaro1,2

2 Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Via Cintia 4, I-80126, Italy
3 Istituto per la Chimica e la Tecnologia dei Materiali Polimerici – ICTMP – CNR, Catania, Italy
4 Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
5 Institute for Cell and Molecular Biosciences, The Medical School
6 Department of Medical Microbiology
7 Cardiopulmonary Transplantation, Freeman Hospital
8 Applied Immunobiology and Transplantation Group, Institute of Cellular Medicine, University of Newcastle, Newcastle, UK

1 To whom correspondence should be addressed: Tel: +39-081-674123; Fax: +39-081-674393; e-mail: molinaro{at}unina.it

Received on May 30, 2008; revised on July 28, 2008; accepted on July 29, 2008

The Burkholderia cepacia complex is a group of Gram-negative bacteria that are opportunistic pathogens for humans especially in cystic fibrosis patients. Lipopolysaccharide (LPS) molecules are potent virulence factors of Gram-negative bacteria organisms essential for bacterial survival. A complete analysis of the bacterial lipopolysaccharide structure to function relationship is required to understand the chemical basis of the inflammatory process. We have therefore investigated the structures of lipopolysaccharides from clonally identical Burkholderia multivorans strains (genomovar II) isolated pre- and post-lung transplantation through compositional analysis, mass spectrometry, and 2D NMR spectroscopy. We tested the LPS proinflammatory activity as a stimulant of human myelomonocytic U937 cell cytokine induction and assessed TLR4/MD2 signaling. Marked changes between the paired strains were found in the lipid A-inner core region. Such structural variations can contribute to the bacterial survival and persistence of infections despite the loss of a CF milieu following lung transplantation.

Key words: Burkholderia multivorans / cystic fibrosis / lipooligosaccharide / lung transplantation / NMR spectroscopy


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