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Glycobiology Advance Access originally published online on October 27, 2007
Glycobiology 2008 18(1):97-103; doi:10.1093/glycob/cwm116
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization and Protection on Acute Liver Injury of a Polysaccharide MP-I from Mytilus Coruscus

Hongli Xu2,3, Tingting Guo4, Yi-feng Guo2, Jianp eng Zhang2, Yang Li2, Weihua Feng1,2 and Binghua Jiao1,2

2 Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433
3 Department of Epidemiology, Cancer Institute of Shanghai Jiaotong University, Shanghai Cancer Institute, Shanghai 200032, China
4 Central Laboratory, Aqua-life Science and Technology College, Shanghai Fisheries University, Shanghai 200090, People's Republic of China

1 To whom correspondence should be addressed to Weihua Feng: Tel: 0086-021-25074233-8007; Fax: 0086-021-65334344; e-mail: fwh402{at}163.com and Binghua Jiao: Tel: 0086-021-25070306-8001; Fax: 0086-021-65334344; e-mail: jiaobh{at}uninet.cn

Received on August 7, 2007; revised on October 11, 2007; accepted on October 12, 2007

In this study, we analyzed a water-soluble polysaccharide MP-I isolated from Mytilus coruscus. MP-I was obtained by hot-water extraction, anion-exchange and gel-permeation chromatography. Complete hydrolysis, periodate oxidation, methylation analysis, as well as Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy were conducted to elucidate its structure. MP-I was subjected to investigate the protective effect on carbon tetrachloride (CCl4) induced liver damage in male Kunming mice. Based on the data obtained, MP-I was found to be an {alpha}-(1->4)-D-glucan, branched with a single {alpha}-D-glucose at the C-6 position every eight residue, on average, along the main chain. Based on the calibration with Dextran, the glucan had a molecular weight of about 1.35 x 106 Da. Pharmacological studies revealed that MP-I could decrease serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), and hepatic malondialdehyde aldehydes (MDA) levels, increase the hepatic total superoxide dismutase (T-SOD) activity, and improve hepatic damage in the CCl4 induced liver injury in mice in a dose-dependent manner. The results suggest that the possible mechanism is due to its antioxidant activity of MP-I.

Key words: antioxidant / carbon tetrachloride / liver injury / Mytilus coruscus polysaccharide I / structure


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