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Glycobiology Advance Access originally published online on October 27, 2007
Glycobiology 2008 18(1):53-65; doi:10.1093/glycob/cwm121
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Overexpression of the 3'-Phosphoadenosine 5'-Phosphosulfate (PAPS) Transporter 1 Increases Sulfation of Chondroitin Sulfate in the Apical Pathway of MDCK II Cells

Gunnar Dick2, Frøy Grøndahl2 and Kristian Prydz1,2

2 Department of Molecular Biosciences, University of Oslo, Box 1041 Blindern, 0316 Oslo, Norway

1 To whom correspondence should be addressed: Tel: +47-22856753; Fax: +47-22854443; e-mail: kristian.prydz{at}imbv.uio.no

Received on July 13, 2007; revised on October 3, 2007; accepted on October 24, 2007

The canine 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 fused to GFP was stably expressed with a typical Golgi localization in MDCK II cells (MDCK II-PAPST1). The capacity for PAPS uptake into Golgi vesicles was enhanced to almost three times that of Golgi vesicles isolated from untransfected cells. We have previously shown that chondroitin sulfate proteoglycans (CSPGs) are several times more intensely sulfated in the basolateral than the apical secretory pathway in MDCK II cells (Tveit H, Dick G, Skibeli V, Prydz K. 2005. A proteoglycan undergoes different modifications en route to the apical and basolateral surfaces of Madin-Darby canine kidney cells. J Biol Chem. 280:29596–29603). Here we demonstrate that increased availability of PAPS in the Golgi lumen enhances the sulfation of CSPG in the apical pathway several times, while sulfation of CSPGs in the basolateral pathway shows minor changes. Sulfation of heparan sulfate proteoglycans is essentially unchanged. Our data indicate that CSPG sulfation in the apical pathway of MDCK II cells occurs at suboptimal conditions, either because the sulfotransferases involved have high Km values, or there is a lower PAPS concentration in the lumen of the apical secretory route than in the basolateral counterpart.

Key words: Golgi apparatus / MDCK epithelial cells / PAPS / proteoglycan / sulfation


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