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Glycobiology Advance Access originally published online on June 22, 2007
Glycobiology 2007 17(9):906-912; doi:10.1093/glycob/cwm059
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

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Intracellular sorting of galectin-8 based on carbohydrate fine specificity

Susanne Carlsson1,, Michael C Carlsson and Hakon Leffler1,

Institute of Laboratory Medicine, Section of Microbiology Immunology and Glycobiology (MIG), Lund University, Sölvegatan 23, S-223 62 Lund, Sweden

1 To whom correspondence should be addressed: Tel: +46-46-173273; Fax: +46-46-137468; e-mail: susanne.carlsson{at}med.lu.se or hakon.leffler{at}med.lu.se

Received on March 5, 2007; revised on May 16, 2007; accepted on May 31, 2007

Galectin-8 has two carbohydrate recognition domains (CRDs), both of which bind ß-galactosides, but have different fine specificity for larger saccharides. Previously we found that both CRDs were needed for efficient cell surface binding and signaling by soluble galectin-8, but unexpectedly binding of the N-CRD to its best ligands, {alpha}2-3-sialylated galactosides, was not needed. In search for another role for this fine specificity, we now compared endocytosis of galectin-8 in Chinese hamster ovary (CHO) cells and in a mutant (Lec2) lacking sialylated glycans, by fluorescence microscopy. Galectin-8 was endocytosed in both cells by a non-clathrin and non-cholesterol dependent pathway, but surprisingly, the pathway after endocytosis differed dramatically. In wild type (wt) cells, galectin-8 was found along the plasma membrane, near the nucleus, and in small vesicles. In the Lec2 cells, galectin-8 was found in larger vesicles evenly spread in the cell, but not along the plasma membrane or near the nucleus. A galectin-8 mutant with an N-CRD having reduced affinity to sialylated glycans and increased affinity for other glycans, gave a Lec2 like pattern in the wt CHO cells, but a wt pattern in the Lec2 cells. Moreover, the pattern of galectin-3 after endocytosis differed from that of both the wt and mutant galactin-8. These data clearly demonstrate a role of galectin fine specificity for intracellular targeting.

Key words: endocytosis / galectin / intracellular / sialic acid / sorting


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