Skip Navigation


Glycobiology Advance Access originally published online on March 6, 2007
Glycobiology 2007 17(6):646-654; doi:10.1093/glycob/cwm024
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
17/6/646    most recent
cwm024v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Parry, S.
Right arrow Articles by Dell, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parry, S.
Right arrow Articles by Dell, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Integrated mass spectrometric strategy for characterizing the glycans from glycosphingolipids and glycoproteins: direct identification of sialyl Lex in mice

Simon Parry2, Victoria Ledger2, Bérangère Tissot2, Stuart M. Haslam2, James Scott3, Howard R. Morris2,4 and Anne Dell1,2

2 Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK
3 Imperial College Genetics and Genomics Research Institute, London SW7 2AZ, UK
4 M-SCAN Ltd, Wokingham, Berks RG41 2TZ, UK

1 To whom correspondence should be addressed; Tel: +44 2075945219; fax: +44 2072250458; e-mail: a.dell{at}imperial.ac.uk

Received on December 14, 2006; revised on February 21, 2007; accepted on February 22, 2007

The current interest in applying systems biology approaches to studying an organism's form or function promises to reveal further insights into the role of glycosylation in cells and whole organisms. This has prompted the development of a rapid, sensitive method of profiling the glycan component of both glycosphingolipids and glycoproteins from a single sample. Here we report a new mass spectrometric screening strategy for characterizing glycosphingolipid-derived oligosaccharides, which can be integrated into an existing highly sensitive glycoprotein glycomics strategy. Using ceramide glycanase to release the glycans from glycosphingolipids, this method provides a reliable profile of the glycosphingolipid-derived glycans present in a sample and has revealed new glycan structures. Glycoproteins are also efficiently recovered using this method, allowing the subsequent analysis of glycoprotein-derived glycans by mass spectrometry. The high sensitivity of this glycomic screening method allowed us to directly characterize the sialyl Lex epitope from mouse brain for the first time, where it was observed on an O-mannose structure. Thus, we present a mass spectrometric method that allows glycomic screening of N- and O-glycans as well as glycosphingolipid-derived glycans from a single tissue.

Key words: glycan / glycosphingolipid / glycoprotein / mass spectrometry / method


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
R. W. Y. Chan, M. C. W. Chan, A. C. N. Wong, R. Karamanska, A. Dell, S. M. Haslam, A. D. L. Sihoe, W. H. Chui, G. Triana-Baltzer, Q. Li, et al.
DAS181 Inhibits H5N1 Influenza Virus Infection of Human Lung Tissues
Antimicrob. Agents Chemother., September 1, 2009; 53(9): 3935 - 3941.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
B. Tissot, N. Gasiunas, A. K Powell, Y. Ahmed, Z.-l. Zhi, S. M Haslam, H. R Morris, J. E Turnbull, J. T Gallagher, and A. Dell
Towards GAG glycomics: Analysis of highly sulfated heparins by MALDI-TOF mass spectrometry
Glycobiology, September 1, 2007; 17(9): 972 - 982.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.