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Glycobiology Advance Access originally published online on September 21, 2007
Glycobiology 2007 17(12):1284-1298; doi:10.1093/glycob/cwm093
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Molecular and Biological Characterization of a Mannan-Binding Lectin from the Holothurian Apostichopus Japonicus

Aleksandr A Bulgakov1,2, Marina G Eliseikina3, Irina Yu Petrova3, Evgeny L Nazarenko2, Svetlana N Kovalchuk2, Valery B Kozhemyako2 and Valery A Rasskazov2

2 Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Science, Vladivostok 690041, Russia
3 Institute of Marine Biology, Far Eastern Branch, Russian Academy of Science, Vladivostok 690041, Russia

1 To whom correspondence should be addressed: Tel: +7-4234-310719; Fax: +7-4234-314050; e-mail: aabulgakov{at}yahmo.com

Received on January 15, 2007; revised on August 30, 2007; accepted on August 31, 2007

To elucidate the origin and evolution of mannan-binding lectins (MBL), a new C-type lectin (CTL) specific for high-mannose glycans (MBL-AJ) was isolated from the coelomic plasma of the holothurian Apostichopus japonicus. MBL-AJ has oligomeric forms with identical 17-kDa subunits on SDS-PAGE. Among natural ligands, lectin hemagglutination activity was competitively inhibited by extracellular low-branched, but not high-branched, {alpha}-D-mannans isolated from marine halophilic bacteria and composed of {alpha}-1,2 and {alpha}-1,6 linked D-mannose residues. This suggests that the lectin interacts with backbone or inner side chain mannose residues, but not with terminal ones. The activity of the lectin was Ca2+-, pH-, and temperature-dependent. MBL-AJ cDNA was cloned from a holothurian coelomocyte cDNA library. The subunit of the mature protein has 159 amino acids and a single carbohydrate-recognition domain (CRD) of CTL. CRD contains a Glu-Pro-Asp amino acid sequence (EPN-motif) conserved for all known MBLs. A monospecific polyclonal antibody against MBL-AJ was obtained using the 34-kDa lectin dimer as an immunogen. The MBL-AJ has demonstrated immunochemical identity to the earlier isolated mannan-binding CTL from another holothurian, Cucumaria japonica. But a more interesting finding was cross-reactivity of MBL-AJ and human serum MBL detected by the antibody against MBL-AJ. Taking into consideration such MBL-AJ peculiarities as its carbohydrate specificity, the presence of a conserved region forming the mannose-binding site, common antigenic determinants with human MBL, and participation in defense reactions, it is possible that MBL-AJ belongs to the family of evolutionary conserved mannan-binding proteins.

Key words: Apostichopus japonicus / bacterial mannans / echinoderms immunity / mannan-binding lectins


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