Glycobiology Advance Access originally published online on September 14, 2006
Glycobiology 2007 17(1):1-9; doi:10.1093/glycob/cwl047
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Mice lacking 
1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors
2 Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan
3 Department of Anatomy and Embryology, Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
4 Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan
5 Division of Oncological Pathology, Aichi Cancer Centre Research Institute, Chikusa-ku, Nagoya, Aichi 464-0021, Japan
6 Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577, Japan
7 Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan
1 To whom correspondence should be addressed; Tel: +81-29-861-3200; Fax: +81-29-861-3201; e-mail: h.narimatsu{at}aist.go.jp
Received on February 8, 2006; revised on August 26, 2006; accepted on September 5, 2006
The 3-fucosyl-N-acetyllactosamine [Lewis x (Lex), CD15, SSEA-1] carbohydrate structure is expressed on several glycolipids, glycoproteins, and proteoglycans of the nervous system and has been implicated in cell–cell recognition, neurite outgrowth, and neuronal migration during development. To characterize the functional role of Lex carbohydrate structure in vivo, we have generated mutant mice that lack 
1,3-fucosyltransferase IX (Fut9–/–). Fut9–/– mice were unable to synthesize the Lex structure carried on glycoproteins and glycolipids in embryonic and adult brain. However, no obvious pathological differences between wild-type and Fut9–/– mice were found in brain. In behavioral tests, Fut9–/– mice exhibited increased anxiety-like responses in dark–light preference and in elevated plus maze tests. Immunohistochemical analysis showed that the number of calbindin-positive neurons was decreased in the basolateral amygdala in Fut9–/– mice. These observations indicated that the carbohydrates synthesized by Fut9 play critical roles in functional regulations of interneurons in the amygdalar subdivisions and suggested a role for the Lex structure in some aspects of emotional behavior in mice.
Key words:
1,3-fucosyltransferase
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Lewis x
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knockout mouse
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anxiety
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amygdala
None declared.
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