Skip Navigation


Glycobiology Advance Access originally published online on May 25, 2006
Glycobiology 2006 16(9):777-785; doi:10.1093/glycob/cwl005
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
16/9/777    most recent
cwl005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ikehara, Y.
Right arrow Articles by Narimatsu, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ikehara, Y.
Right arrow Articles by Narimatsu, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Apical Golgi localization of N,N'-diacetyllactosediamine synthase, ß4GalNAc-T3, is responsible for LacdiNAc expression on gastric mucosa

Yuzuru Ikehara2, Takashi Sato3, Toru Niwa2, Sachiko Nakamura4, Masanori Gotoh3, Sanae Kabata Ikehara2, Katsue Kiyohara3, Chihiro Aoki2, Toshie Iwai3, Hayao Nakanishi2, Jun Hirabayashi4, Masae Tatematsu2, and Hisashi Narimatsu1,3

2 Division of Oncological Pathology, Aichi Cancer Center Research Institute; 1-1 Kanokoden, Chiksa-ku, Nagoya 464–8681, Japan; 3 Glycogene Function Team and 4 Glycostructure Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki 305–8568, Japan

1 To whom correspondence should be addressed; e-mail: h.narimatsu{at}aist.go.jp

Received on October 26, 2005; revised on May 15, 2006; accepted on May 22, 2006

ß1,4-N-acetylgalactosaminyltransferase III (ß4GalNAc-T3), which was recently cloned and identified, exhibits GalNAc transferase activity toward a GlcNAcß residue with ß1,4-linkage, forming the N,N'-diacetyllactosediamine, GalNAcß1,4GlcNAc (LacdiNAc or LDN). Though LacdiNAc has not been found in the gastric mucosa, a large amount of transcript was detected in our previous study. To increase our knowledge of ß4GalNAc-T3 expression and its product LacdiNAc, we examined the exact localization of ß4GalNAc-T3 in human gastric mucosa using a newly developed antibody, monoclonal antibody (mAb) K1356. This antibody specifically detected the enzyme that transfected the ß4GalNAc-T3 gene into MKN45 cells, and the terminal ßGalNAc epitope yielded on the cell surface was recognized by a lectin, Wisteria floribunda agglutinin (WFA). ß4GalNAc-T3 was localized in the supra-nuclear region of surface mucous cells in gastric mucosa, and WFA positively stained the mucins secreted by the cells. In contrast, in the cells of the glandular compartment in the fundic glands and a few cells in the pyloric glands, ß4GalNAc-T3 was observed in the basolateral position of the nucleus, where no WFA reactivity was detected. The anti-Tn (GalNAc{alpha}-O-Ser/Thr) antibody staining did not overlap with the WFA staining. By measuring the binding activity of WFA using automated frontal affinity chromatography (FAC), we found WFA to bind most strongly LacdiNAc among the sugar chains examined. Neither ß4GalNAc-T3 nor WFA-positive staining was detected in intestinal metaplastic cells. These results suggest that the supra-nuclear expression of ß4GalNAc-T3 is essential for the formation of LacdiNAc on the surface mucous cells and that LacdiNAc and ß4GalNAc-T3 are novel differentiation markers of surface mucous cells in the gastric mucosa.

Key words: gastric mucosa / N,N'-diacetyllactosediamine / ß4GalNAc-T3 expression


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. Miller, D. Fiete, N. M. J. Blake, M. Beranek, E. L. Oates, Y. Mi, D. S. Roseman, and J. U. Baenziger
A Necessary and Sufficient Determinant for Protein-selective Glycosylation in Vivo
J. Biol. Chem., January 25, 2008; 283(4): 1985 - 1991.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.