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Glycobiology Advance Access originally published online on February 20, 2006
Glycobiology 2006 16(6):538-550; doi:10.1093/glycob/cwj094
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Structure of the lipid A–inner core region and biological activity of Plesiomonas shigelloides O54 (strain CNCTC 113/92) lipopolysaccharide

Jolanta Lukasiewicz1,2, Tomasz Niedziela2, Wojciech Jachymek2, Lennart Kenne3 and Czeslaw Lugowski2

2 Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland; and 3 Department of Chemistry, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden

1 To whom correspondence should be addressed; e-mail: czaja{at}iitd.pan.wroc.pl

Received on January 19, 2006; revised on February 15, 2006; accepted on February 16, 2006

Plesiomonas shigelloides is a Gram-negative rod associated with episodes of intestinal infections and outbreaks of diarrhea in humans. The extraintestinal infections caused by this bacterium, for example, endopthalmitis, meningitidis, bacteremia, and septicemia, usually have gastrointestinal origin and serious course. The lipopolysaccharide (LPS, endotoxin) as virulence factor is important in enteropathogenicity of this bacterium. LPSs of P. shigelloides and especially their lipid A part, that is, the immunomodulatory center of LPS, have not been extensively investigated. The structure of P. shigelloides O54 lipid A was determined by chemical analysis combined with MALDI-TOF mass spectrometry, and the intact Kdo-containing core region was investigated by NMR spectroscopy on deacylated LPS. Products from alkaline deacylation of LPS, containing 4-substituted uronic acids, are usually very complex and difficult to separate. Since Kdo residues, like sialic acids, form complexes with serotonin, we used immobilized serotonin for one-step isolation of oligosaccharide containing the intact Kdo region from the reaction mixture by affinity chromatography. The major form of lipid A was built of ß-D-GlcpN4PPEtn-(1->6)-{alpha}-D-GlcpN1P disaccharide substituted with 14:0(3-OH), 12:0(3-OH), 14:0(3-O-14:0), and 12:0(3-O-12:0) acyl groups at N-2, O-3, N-2', and O-3', respectively. This is a novel structure among known lipid A molecules. Analysis of intact Kdo-lipid A region, lipid A and its linkage with the core oligosaccharide completes the structural investigation of P. shigelloides O54 LPS, resolving the entire molecule. Biological activities and observed discrepancy between in vitro and in vivo activity of P. shigelloides and Escherichia coli LPS are discussed.

Key words: lipid A / lipopolysaccharide / MALDI-TOF mass spectrometry / NMR spectroscopy / Plesiomonas shigelloides


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Home page
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J. Lukasiewicz, T. Niedziela, W. Jachymek, L. Kenne, and C. Lugowski
Two Kdo-Heptose Regions Identified in Hafnia alvei 32 Lipopolysaccharide: the Complete Core Structure and Serological Screening of Different Hafnia O Serotypes
J. Bacteriol., January 15, 2009; 191(2): 533 - 544.
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