Glycobiology Advance Access originally published online on July 21, 2005
Glycobiology 2006 16(2):29R-37R; doi:10.1093/glycob/cwj016
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
REVIEW |
Structures and mechanisms of glycosyltransferases
najdrová2,3
a32 CERMAV–CNRS, affiliated with Université Joseph Fourier, PO Box 53, 38041 Grenoble cedex 9, France; and 3 NCBR, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic
1 To whom correspondence should be addressed; e-mail: breton{at}cermav.cnrs.fr and imberty{at}cermav.cnrs.fr
Glycosyltransferases (GTs) catalyze the transfer of a sugar moiety from an activated donor sugar onto saccharide and nonsaccharide acceptors. A sequence-based classification spreads GTs in many families thus reflecting the variety of molecules that can be used as acceptors. In contrast, this enzyme family is characterized by a more conserved three-dimensional architecture. Until recently, only two different folds (GT-A and GT-B) have been identified for solved crystal structures. The recent report of a structure for a bacterial sialyltransferase allows the definition of a new fold family. Progress in the elucidation of the structures and mechanisms of GTs are discussed in this review. To accommodate the growing number of crystal structures, we created the 3D-Glycosyltransferase database to gather structural information concerning this class of enzymes.
Key words: fold recognition / glycosyltransferase / mechanism / superfamily / three-dimensional structure
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Barreras, S. R. Salinas, P. L. Abdian, M. A. Kampel, and L. Ielpi Structure and Mechanism of GumK, a Membrane-associated Glucuronosyltransferase J. Biol. Chem., September 5, 2008; 283(36): 25027 - 25035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-J. Cao, L. E. Bartley, K.-H. Jung, and P. C. Ronald Construction of a Rice Glycosyltransferase Phylogenomic Database and Identification of Rice-Diverged Glycosyltransferases Mol Plant, September 1, 2008; 1(5): 858 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. K Shoemaker, N. Soya, M. M Palcic, and J. S Klassen Temperature-dependent cooperativity in donor-acceptor substrate binding to the human blood group glycosyltransferases Glycobiology, August 1, 2008; 18(8): 587 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Brockhausen, B. Hu, B. Liu, K. Lau, W. A. Szarek, L. Wang, and L. Feng Characterization of Two {beta}-1,3-Glucosyltransferases from Escherichia coli Serotypes O56 and O152 J. Bacteriol., July 15, 2008; 190(14): 4922 - 4932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Vetting, P. A. Frantom, and J. S. Blanchard Structural and Enzymatic Analysis of MshA from Corynebacterium glutamicum: SUBSTRATE-ASSISTED CATALYSIS J. Biol. Chem., June 6, 2008; 283(23): 15834 - 15844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Morita, Y. Kizuka, S. Kakuda, and S. Oka Expression and Function of the HNK-1 Carbohydrate J. Biochem., June 1, 2008; 143(6): 719 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Alfaro, R. B. Zheng, M. Persson, J. A. Letts, R. Polakowski, Y. Bai, S. N. Borisova, N. O. L. Seto, T. L. Lowary, M. M. Palcic, et al. ABO(H) Blood Group A and B Glycosyltransferases Recognize Substrate via Specific Conformational Changes J. Biol. Chem., April 11, 2008; 283(15): 10097 - 10108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Chua, J. M. Bujnicki, T.-C. Tan, F. Huynh, B. K. Patel, and J. Sivaraman The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode PLANT CELL, April 1, 2008; 20(4): 1059 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xiong, A.-S. Patana, M. J. Miley, A. K. Zielinska, S. M. Bratton, G. P. Miller, A. Goldman, M. Finel, M. R. Redinbo, and A. Radominska-Pandya The First Aspartic Acid of the DQxD Motif for Human UDP-Glucuronosyltransferase 1A10 Interacts with UDP-Glucuronic Acid during Catalysis Drug Metab. Dispos., March 1, 2008; 36(3): 517 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jank, T. Giesemann, and K. Aktories Clostridium difficile Glucosyltransferase Toxin B-essential Amino Acids for Substrate Binding J. Biol. Chem., November 30, 2007; 282(48): 35222 - 35231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Alvarez-Manilla, N. L. Warren, T. Abney, J. Atwood III, P. Azadi, W. S York, M. Pierce, and R. Orlando Tools for glycomics: relative quantitation of glycans by isotopic permethylation using 13CH3I Glycobiology, July 1, 2007; 17(7): 677 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Moretti and J. S. Thorson Enhancing the Latent Nucleotide Triphosphate Flexibility of the Glucose-1-phosphate Thymidylyltransferase RmlA J. Biol. Chem., June 8, 2007; 282(23): 16942 - 16947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Berg, D. Kaur, M. Jackson, and P. J Brennan The glycosyltransferases of Mycobacterium tuberculosis--roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates Glycobiology, June 1, 2007; 17(6): 35R - 56R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Xayarath and J. Yother Mutations Blocking Side Chain Assembly, Polymerization, or Transport of a Wzy-Dependent Streptococcus pneumoniae Capsule Are Lethal in the Absence of Suppressor Mutations and Can Affect Polymer Transfer to the Cell Wall J. Bacteriol., May 1, 2007; 189(9): 3369 - 3381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Persson, J. A. Letts, B. Hosseini-Maaf, S. N. Borisova, M. M. Palcic, S. V. Evans, and M. L. Olsson Structural Effects of Naturally Occurring Human Blood Group B Galactosyltransferase Mutations Adjacent to the DXD Motif J. Biol. Chem., March 30, 2007; 282(13): 9564 - 9570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O'Neill, and T. Ishii A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose Glycobiology, March 1, 2007; 17(3): 345 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhang, B. R. Griffith, Q. Fu, C. Albermann, X. Fu, I.-K. Lee, L. Li, and J. S. Thorson Exploiting the reversibility of natural product glycosyltransferase-catalyzed reactions. Science, September 1, 2006; 313(5791): 1291 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. T. Larson, D. Reiter, M. Young, and C. M. Lawrence Structure of A197 from Sulfolobus Turreted Icosahedral Virus: a Crenarchaeal Viral Glycosyltransferase Exhibiting the GT-A Fold. J. Virol., August 1, 2006; 80(15): 7636 - 7644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Liu and M. E. Tanner PseG of Pseudaminic Acid Biosynthesis: A UDP-SUGAR HYDROLASE AS A MASKED GLYCOSYLTRANSFERASE J. Biol. Chem., July 28, 2006; 281(30): 20902 - 20909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Ciocchini, M. S. Roset, G. Briones, N. I. de Iannino, and R. A. Ugalde Identification of active site residues of the inverting glycosyltransferase Cgs required for the synthesis of cyclic {beta}-1,2-glucan, a Brucella abortus virulence factor Glycobiology, July 1, 2006; 16(7): 679 - 691. [Abstract] [Full Text] [PDF]
|
|