Skip Navigation


Glycobiology Advance Access originally published online on October 19, 2005
Glycobiology 2006 16(2):117-131; doi:10.1093/glycob/cwj048
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/2/117    most recent
cwj048v2
cwj048v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (47)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Comelli, E. M.
Right arrow Articles by Paulson, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Comelli, E. M.
Right arrow Articles by Paulson, J. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

A focused microarray approach to functional glycomics: transcriptional regulation of the glycome

Elena M. Comelli1,3,4, Steven R. Head1,5, Tim Gilmartin5, Thomas Whisenant5, Stuart M. Haslam6, Simon J. North6, Nyet-Kui Wong6, Takashi Kudo7, Hisashi Narimatsu7, Jeffrey D. Esko8, Kurt Drickamer6, Anne Dell6 and James C. Paulson2,4

4 Department of Molecular Biology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037; 5 DNA Microarray Core Facility, The Scripps Research Institute, La Jolla, CA 92037; 6 Imperial College London, South Kensington Campus, London SW7 2AZ, UK; 7 AIST, Tsukuba 305–8568, Japan; and 8 University of California San Diego, La Jolla, CA 92037

1 These authors contributed equally to this work.

2 To whom correspondence should be addressed; e-mail: jpaulson{at}scripps.edu

3 Present address: Nestlé Research Centre, Department of Nutrition and Health, Vers chez les Blanc, 1000 Lausanne 26, Switzerland

Received on July 15, 2005; revised on September 15, 2005; accepted on October 11, 2005

Glycosylation is the most common posttranslational modification of proteins, yet genes relevant to the synthesis of glycan structures and function are incompletely represented and poorly annotated on the commercially available arrays. To fill the need for expression analysis of such genes, we employed the Affymetrix technology to develop a focused and highly annotated glycogene-chip representing human and murine glycogenes, including glycosyltransferases, nucleotide sugar transporters, glycosidases, proteoglycans, and glycan-binding proteins. In this report, the array has been used to generate glycogene-expression profiles of nine murine tissues. Global analysis with a hierarchical clustering algorithm reveals that expression profiles in immune tissues (thymus [THY], spleen [SPL], lymph node, and bone marrow [BM]) are more closely related, relative to those of nonimmune tissues (kidney [KID], liver [LIV], brain [BRN], and testes [TES]). Of the biosynthetic enzymes, those responsible for synthesis of the core regions of N- and O-linked oligosaccharides are ubiquitously expressed, whereas glycosyltransferases that elaborate terminal structures are expressed in a highly tissue-specific manner, accounting for tissue and ultimately cell-type-specific glycosylation. Comparison of gene expression profiles with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) profiling of N-linked oligosaccharides suggested that the {alpha}1-3 fucosyltransferase 9, Fut9, is the enzyme responsible for terminal fucosylation in KID and BRN, a finding validated by analysis of Fut9 knockout mice. Two families of glycan-binding proteins, C-type lectins and Siglecs, are predominately expressed in the immune tissues, consistent with their emerging functions in both innate and acquired immunity. The glycogene chip reported in this study is available to the scientific community through the Consortium for Functional Glycomics (CFG) (http://www.functionalglycomics.org).

Key words: Fut9 / glycomics / glycosyltransferase / lectin / microarray


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. L. Montpetit, P. J. Stocker, T. A. Schwetz, J. M. Harper, S. A. Norring, L. Schaffer, S. J. North, J. Jang-Lee, T. Gilmartin, S. R. Head, et al.
Regulated and aberrant glycosylation modulate cardiac electrical signaling
PNAS, September 22, 2009; 106(38): 16517 - 16522.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
F. Guillerme-Bosselut, L. Forestier, C. Jayat-Vignoles, J.-L. Vilotte, I. Popa, J. Portoukalian, A. Le Dur, H. Laude, R. Julien, and P.-F. Gallet
Glycosylation-related gene expression profiling in the brain and spleen of scrapie-affected mouse
Glycobiology, August 1, 2009; 19(8): 879 - 889.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Nairn, W. S. York, K. Harris, E. M. Hall, J. M. Pierce, and K. W. Moremen
Regulation of Glycan Structures in Animal Tissues: TRANSCRIPT PROFILING OF GLYCAN-RELATED GENES
J. Biol. Chem., June 20, 2008; 283(25): 17298 - 17313.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
Z. S Kawar, T. K Johnson, S. Natunen, J. B Lowe, and R. D Cummings
PSGL-1 from the murine leukocytic cell line WEHI-3 is enriched for core 2-based O-glycans with sialyl Lewis x antigen
Glycobiology, June 1, 2008; 18(6): 441 - 446.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Yagi, M. Nakagawa, N. Takahashi, S. Kondo, M. Matsubara, and K. Kato
Neural complex-specific expression of xylosyl N-glycan in Ciona intestinalis
Glycobiology, February 1, 2008; 18(2): 145 - 151.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. Powlesland, T. Fisch, M. E. Taylor, D. F. Smith, B. Tissot, A. Dell, S. Pohlmann, and K. Drickamer
A Novel Mechanism for LSECtin Binding to Ebola Virus Surface Glycoprotein through Truncated Glycans
J. Biol. Chem., January 4, 2008; 283(1): 593 - 602.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Bax, J. J. Garcia-Vallejo, J. Jang-Lee, S. J. North, T. J. Gilmartin, G. Hernandez, P. R. Crocker, H. Leffler, S. R. Head, S. M. Haslam, et al.
Dendritic Cell Maturation Results in Pronounced Changes in Glycan Expression Affecting Recognition by Siglecs and Galectins
J. Immunol., December 15, 2007; 179(12): 8216 - 8224.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
G. Alvarez-Manilla, N. L. Warren, T. Abney, J. Atwood III, P. Azadi, W. S York, M. Pierce, and R. Orlando
Tools for glycomics: relative quantitation of glycans by isotopic permethylation using 13CH3I
Glycobiology, July 1, 2007; 17(7): 677 - 687.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. Ishii, T. Ikeda, S. Hitoshi, I. Fujimoto, T. Torii, K. Sakuma, S.-i. Nakakita, S. Hase, and K. Ikenaka
Developmental changes in the expression of glycogenes and the content of N-glycans in the mouse cerebral cortex
Glycobiology, March 1, 2007; 17(3): 261 - 276.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
T. Kudo, T. Fujii, S. Ikegami, K. Inokuchi, Y. Takayama, Y. Ikehara, S. Nishihara, A. Togayachi, S. Takahashi, K. Tachibana, et al.
Mice lacking {alpha}1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors
Glycobiology, January 1, 2007; 17(1): 1 - 9.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
R. R. Drake, E. E. Schwegler, G. Malik, J. Diaz, T. Block, A. Mehta, and O. J. Semmes
Lectin Capture Strategies Combined with Mass Spectrometry for the Discovery of Serum Glycoprotein Biomarkers
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1957 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. M. Comelli, M. Sutton-Smith, Q. Yan, M. Amado, M. Panico, T. Gilmartin, T. Whisenant, C. M. Lanigan, S. R. Head, D. Goldberg, et al.
Activation of Murine CD4+ and CD8+ T Lymphocytes Leads to Dramatic Remodeling of N-Linked Glycans
J. Immunol., August 15, 2006; 177(4): 2431 - 2440.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
R. Raman, M. Venkataraman, S. Ramakrishnan, W. Lang, S. Raguram, and R. Sasisekharan
Advancing glycomics: Implementation strategies at the Consortium for Functional Glycomics
Glycobiology, May 1, 2006; 16(5): 82R - 90R.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.