Glycobiology Advance Access originally published online on July 19, 2006
Glycobiology 2006 16(11):1052-1063; doi:10.1093/glycob/cwl024
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Oligosaccharides modulate the apoptotic activity of glycodelin
2 Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India; and 3 Glycobiology Institute, Department of Biochemistry, Oxford University, Oxford OX1 3QU, UK
1 To whom correspondence should be addressed; e-mail: anjali{at}biochem.iisc.ernet.in
Received on March 31, 2006; revised on July 10, 2006; accepted on July 11, 2006
GlycodelinA (GdA), a multifunctional glycoprotein secreted at high concentrations by the uterine endometrium during the early phases of pregnancy, carries glycan chains on asparagines at positions N28 and N63. GdA purified from amniotic fluid is known to be a suppressor of T-cell proliferation, an inducer of T-cell apoptosis, and an inhibitor of sperm–zona binding in contrast to its glycoform, glycodelinS (GdS), which is secreted by the seminal vesicles into the seminal plasma. The oligosaccharide chains of GdA terminate in sialic acid residues, whereas those of GdS are not sialylated but are heavily fucosylated. Our previous work has shown that the apoptogenic activity of GdA resides in the protein backbone, and we have also demonstrated the importance of sialylation for the manifestation of GdA-induced apoptosis. Recombinant glycodelin (Gd) expressed in the Sf21 insect cell line yielded an apoptotically active Gd; however, the same gene expressed in the insect cell line Tni produced apoptotically inactive Gd, as observed with the gene expressed in the Chinese hamster ovary (CHO) cell line and earlier in Pichia pastoris. Glycan analysis of the Tni and Sf21 cell line-expressed Gd proteins reveals differences in their glycan structures, which modulate the manifestation of apoptogenic activity of Gd. Through apoptotic assays carried out with the wild-type (WT) and glycosylation mutants of Gd expressed in Sf21 and Tni cells before and after mannosidase digestion, we conclude that the accessibility to the apoptogenic region of Gd is influenced by the size of the glycans.
Key words: apoptosis / glycans / glycodelin / mannosidase / sialic acids
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