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Glycobiology Advance Access originally published online on November 10, 2004
Glycobiology 2005 15(4):323-333; doi:10.1093/glycob/cwi015
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Glycobiology vol. 15 no. 4 © Oxford University Press 2004; all rights reserved.

Structural analysis of the lipopolysaccharide from Pasteurella multocida genome strain Pm70 and identification of the putative lipopolysaccharide glycosyltransferases

Frank St. Michael, Evgeny Vinogradov, Jianjun Li and Andrew D. Cox1

Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada, K1A 0R6

1 To whom correspondence should be addressed; e-mail: andrew.cox{at}nrc-cnrc.gc.ca

Received on July 13, 2004; revised on October 1, 2004; accepted on November 5, 2004

Pasteurella multocida is an important multispecies veterinary pathogen. The cell surface lipopolysaccharide (LPS) is an important virulence factor and forms the basis of the serotyping scheme, although little structural information about it is known. The structure of the LPS from the Pasteurella multocida genome strain Pm70 was elucidated in this study. The LPS was subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy, and mass spectrometry. The structure of the core oligosaccharide was determined on the basis of the combined data from these experiments. Identification of the core oligosaccharide structure enabled a search for glycosyltransferase homologs in the Pm70 genome and revealed a clustering of the genes putatively responsible for outer core oligosaccharide biosynthesis.

Key words: core oligosaccharide / lipopolysaccharide / mass spectrometry / nuclear magnetic resonance / Pasteurella multocida


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