Skip Navigation


Glycobiology Advance Access originally published online on September 29, 2004
Glycobiology 2005 15(2):193-201; doi:10.1093/glycob/cwh159
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
15/2/193    most recent
cwh159v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Bakker, H.
Right arrow Articles by Bosch, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bakker, H.
Right arrow Articles by Bosch, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology vol. 15 no. 2 © Oxford University Press 2005; all rights reserved.

Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line

Hans Bakker1,2,3,4, Françoise Routier1,4, Stefan Oelmann4, Wilco Jordi3, Arjen Lommen5, Rita Gerardy-Schahn4 and Dirk Bosch3

3 Plant Research International, Wageningen University and Research Centre, P.O.Box 16, 6700AA, Wageningen, The Netherlands; 4 Zelluläre Chemie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany; and 5 Institute of Food Safety (RIKILT), Wageningen University and Research Centre, P.O. Box 230, 6700AE Wageningen, The Netherlands

2 To whom correspondence should be addressed; e-mail: bakker.hans{at}mh-hannover.de

Received on July 16, 2004; revised on September 21, 2004; accepted on September 23, 2004

Nucleotide-sugar transporters (NSTs) form a family of structurally related transmembrane proteins that transport nucleotide-sugars from the cytoplasm to the endoplasmic reticulum and Golgi lumen. In these organelles, activated sugars are substrates for various glycosyltransferases involved in oligo- and polysaccharide biosynthesis. The Arabidopsis thaliana genome contains more than 40 members of this transporter gene family, of which only a few are functionally characterized. In this study, two Arabidopsis UDP-galactose transporter cDNAs (UDP-GalT1 and UDP-GalT2) are isolated by expression cloning using a Chinese hamster ovary cell line (CHO-Lec8) deficient in UDP-galactose transport. The isolated genes show only 21% identity to each other and very limited sequence identity with human and yeast UDP-galactose transporters and other NSTs. Despite this low overall identity, the two proteins clearly belong to the same gene family. Besides complementing Lec8 cells, the two NSTs are shown to transport exclusively UDP-galactose by an in vitro NST assay. The most homologous proteins with known function are plant transporters that locate in the inner chloroplast membrane and transport triose-phosphate, phosphoenolpyruvate, glucose-6-phosphate, and xylulose 5-phosphate. Also, the latter proteins are members of the same family, which therefore has been named the NST/triose-phosphate transporter family.

Key words: cell wall / expression cloning / Golgi / membrane / nucleotide-sugar transporter

1 These authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. Ibar and A. Orellana
The Import of S-Adenosylmethionine into the Golgi Apparatus Is Required for the Methylation of Homogalacturonan
Plant Physiology, October 1, 2007; 145(2): 504 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. R. Cottrell, C. L. Griffith, H. Liu, A. A. Nenninger, and T. L. Doering
The Pathogenic Fungus Cryptococcus neoformans Expresses Two Functional GDP-Mannose Transporters with Distinct Expression Patterns and Roles in Capsule Synthesis
Eukaryot. Cell, May 1, 2007; 6(5): 776 - 785.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-C. Lamerz, T. Haselhorst, A. K. Bergfeld, M. von Itzstein, and R. Gerardy-Schahn
Molecular Cloning of the Leishmania major UDP-glucose Pyrophosphorylase, Functional Characterization, and Ligand Binding Analyses Using NMR Spectroscopy
J. Biol. Chem., June 16, 2006; 281(24): 16314 - 16322.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
R. Kabuss, A. Ashikov, S. Oelmann, R. Gerardy-Schahn, and H. Bakker
Endoplasmic reticulum retention of the large splice variant of the UDP-galactose transporter is caused by a dilysine motif
Glycobiology, October 1, 2005; 15(10): 905 - 911.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ashikov, F. Routier, J. Fuhlrott, Y. Helmus, M. Wild, R. Gerardy-Schahn, and H. Bakker
The Human Solute Carrier Gene SLC35B4 Encodes a Bifunctional Nucleotide Sugar Transporter with Specificity for UDP-Xylose and UDP-N-Acetylglucosamine
J. Biol. Chem., July 22, 2005; 280(29): 27230 - 27235.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.