Glycobiology Advance Access originally published online on August 11, 2005
Glycobiology 2005 15(12):1368-1375; doi:10.1093/glycob/cwj028
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Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage galactose-type C-type lectin-1-deficient mouse embryos
3 Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, and 4 Department of Radiation Oncology, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
1 Dr. Irimura holds stock and conducts research in collaboration with Summit Glycoresearch Inc.
2 To whom correspondence should be addressed; e-mail: irimura{at}mol.f.u-tokyo.ac.jp
Received on June 8, 2005; revised on August 6, 2005; accepted on September 9, 2005
MGL1/CD301a is a C-type lectin that recognizes galactose and N-acetylgalactosamine as monosaccharides and is expressed on limited populations of macrophages and dendritic cells at least in adult mice. In this study, pregnant mice with Mgl1+/– genotype were mated with Mgl1+/– or Mgl1–/– genotype males, and the embryos were used to assess a hypothesis that this molecule plays an important role in the clearance of apoptotic cells. After X-ray irradiation at 1 Gy of developing embryos at 10.5 days post coitus (d.p.c.), the number of Mgl1–/– pups was significantly reduced as compared with Mgl1+/+ pups. Distributions of MGL1-positive cells, MGL2-positive cells, and apoptotic cells were histologically examined in irradiated Mgl1+/+ embryos. MGL1-positive cells were detected in the neural tube in which many cells undergo apoptosis, whereas MGL2-positive cells were not observed. Biotinylated recombinant MGL1 bound a significant portion of the apoptotic cells. When Mgl1+/+ and Mgl1–/– embryos were examined for the presence of apoptotic cells, similar numbers of apoptotic cells gave rise, but the clearance of these cells was slower in Mgl1–/– embryos than in Mgl1+/+ embryos. These results strongly suggest that MGL1/CD301a is involved in the clearance of apoptotic cells. This process should be essential in the repair and normal development of X-ray-irradiated embryos.
Key words: apoptosis / galactose / lectin / macrophage / phagocytosis
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