Glycobiology Advance Access originally published online on June 8, 2005
Glycobiology 2005 15(10):982-993; doi:10.1093/glycob/cwi090
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Developmental and regional expression of heparan sulfate sulfotransferase genes in the mouse brain
Department of Developmental Neuroscience, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan
1 To whom correspondence should be addressed; e-mail: maedan{at}tmin.ac.jp
2 Present address: Neuropsychiatry Research Unit, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N5E4
Received on March 14, 2005; revised on May 24, 2005; accepted on June 5, 2005
Heparan sulfate (HS) binds with various proteins including growth factors, morphogens, and extracellular matrix molecules to regulate their biological functions. These regulatory interactions are considered to be dependent on the structure of HS, which is determined by HS sulfotransferases. To gain insights into the functions of HS sulfotransferases in the development of the nervous system, we examined the expression of these enzymes (3-O-sulfotransferase-1 [3-OST-1], -2, -4; 6-OST-1, -2, -3; and N-deacetylase /N-sulfotransferase-1 [NDST-1], -2, -3) by in situ hybridization and real-time reverse transcription–polymerase chain reaction (RT–PCR). The expression of these genes was spatiotemporally regulated. In the E16 cerebrum, the expression of these genes showed two patterns: (1) selective expression at cortical plate (CP) and ventricular zone (VZ) and (2) wider expression by the cells in the marginal zone (MZ), CP, subplate (SP), and VZ. At P1, most genes showed similar expression patterns, but after P7, these genes were expressed differentially in a layer-specific manner. In the P1 cerebellum, the external granule cell layer (EGL) expressed most genes, the expressions of which were down-regulated at P7. In contrast, Purkinje cells began to express many of these genes after P7. These complex expression patterns suggest that the structure of HS is altered spatiotemporally for regulating various biological activities in the developing brain including the proliferation of neuronal progenitors, extension of axons, and formation of dendrites. We discuss possible functional roles of these sulfotransferases in the signaling of several HS-binding proteins such as fibroblast growth factors, slit, netrin, and sonic hedgehog.
Key words: brain development / heparan sulfate sulfotransferase / in situ hybridization / real-time RT–PCR
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Hu, C. Wang, Y. Xiao, N. Sheng, Y. Chen, Y. Xu, L. Zhang, W. Mo, N. Jing, and G. Hu NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development J. Cell Sci., April 15, 2009; 122(8): 1145 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Abramsson, S. Kurup, M. Busse, S. Yamada, P. Lindblom, E. Schallmeiner, D. Stenzel, D. Sauvaget, J. Ledin, M. Ringvall, et al. Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development Genes & Dev., February 1, 2007; 21(3): 316 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Manzini, M. S. Ward, Q. Zhang, M. D. Lieberman, and C. A. Mason The stop signal revised: immature cerebellar granule neurons in the external germinal layer arrest pontine mossy fiber growth. J. Neurosci., May 31, 2006; 26(22): 6040 - 6051. [Abstract] [Full Text] [PDF]
|
|