Skip Navigation


Glycobiology Advance Access originally published online on June 2, 2005
Glycobiology 2005 15(10):895-904,; doi:10.1093/glycob/cwi084
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
15/10/895    most recent
cwi084v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Pinto, M. R.
Right arrow Articles by Barreto-Bergter, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pinto, M. R.
Right arrow Articles by Barreto-Bergter, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Published by Oxford University Press 2005.

Structures of the O-linked oligosaccharides of a complex glycoconjugate from Pseudallescheria boydii

Marcia R. Pinto2, Philip A.J. Gorin3, Robin Wait4, Barbara Mulloy5 and Eliana Barreto-Bergter1,2

2 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Ilha do Fundão, Rio de Janeiro, RJ 21941-970, Brazil; 3 Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná (UFPR), Curitiba, PR 81531-990, Brazil; 4 Faculty of Medicine, Kennedy Institute of Rheumatology Division, Imperial College London, 1, Aspenlea Road, Hammersmith, London W6 8LH, UK; and 5 Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK

1 To whom correspondence should be addressed; e-mail: eliana.bergter{at}micro.ufrj.br

Received on November 8, 2004; revised on May 20, 2005; accepted on May 23, 2005

Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline ß-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was characterized by a combination of techniques including electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI MS/MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), nuclear magnetic resonance (NMR), and methylation gas–liquid chromatography-mass spectrometry (GC-MS) analysis. It was branched, with a principal chain of {alpha}-Rhap-(1 -> 3)-{alpha}-Rhap-(1 -> 3)-{alpha}-Manp-(1 -> 2)-Man-ol substituted at O-6 of mannitol with an {alpha}-Glcp-(1 -> 4)-ß-Galp group. Species containing one and two additional {alpha}-Glcp-(1 -> 4) substituents in the rhamnose branch were also present. The major component of fraction 2 was a substructure of oligosaccharide-1, lacking a hexose from the Glc-Gal branch. Fraction 3 contained a mixture of smaller, unbranched, oligosaccharides. In hapten inhibition tests, fractions 1 and 2 blocked the reaction between peptidorhamnomannan (PRM) and rabbit anti-P. boydii mycelium hyperimmune serum by ~75%, whereas fraction 3 inhibited by ~55%.

Key words: ELISA / ESI MS / MS / MALDI MS / methylation-GC-MS / Pseudallescheria boydii mycelia


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CVIHome page
C. R. Thornton
Tracking the Emerging Human Pathogen Pseudallescheria boydii by Using Highly Specific Monoclonal Antibodies
Clin. Vaccine Immunol., May 1, 2009; 16(5): 756 - 764.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.