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Glycobiology Advance Access originally published online on June 15, 2005
Glycobiology 2005 15(10):14C-18C; doi:10.1093/glycob/cwi093
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

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The size of the C-chain maltosaccharide of glycogen: evidence for the presence of only a single branch

Soledad Bazán and Juan A. Curtino1

Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), UNC-CONICET, Departamento de Química Biológica DrRanwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina

1 To whom correspondence should be addressed; e-mail: jcurtino{at}dqb.fcq.unc.edu.ar

Received on December 28, 2004; revised on June 8, 2005; accepted on June 10, 2005

Glycogen is found in mammals and yeast bound to glycogenin forming proteoglycogen. The branched polysaccharide is joined to the protein through the C-chain, a maltosaccharide considered to be 13 glucose units long and double branched as the other branched glycogen B-chains. We described before the isolation of c-glycogenin, the debranched C-chain bound to glycogenin, from muscle proteoglycogen. In this work, the size of the C-chain is analyzed for the first time. The maltosaccharide moiety of c-glycogenin was auto[14C]glucosylated by a short incubation with UDP-[14C]glucose, and the labeled maltosaccharide was released by heating in 2 M NaOH containing 0.1 M NaBH4 and analyzed by high-performance thin layer chromatography (HPTLC). The results indicate that the C-chain is about half the size of the B-chains, not long enough to be double branched.

Key words: C-chain-bound glycogenin / c-glycogenin / glycogen structure / glycogenin-bound C-chain / proteoglycogen


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