Skip Navigation


Glycobiology Advance Access originally published online on June 23, 2004
Glycobiology 2004 14(10):931-938; doi:10.1093/glycob/cwh115
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
14/10/931    most recent
cwh115v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ouskova, G.
Right arrow Articles by Prehm, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ouskova, G.
Right arrow Articles by Prehm, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology vol. 14 no. 10 © Oxford University Press 2004; all rights reserved.

Hyaluronan release from Streptococcus pyogenes: export by an ABC transporter

Galina Ouskova2, Barbara Spellerberg3 and Peter Prehm1,2

2 Muenster University Hospital, Institute of Physiological Chemistry and Pathobiochemistry, Waldeyerstr. 15, D-48129, Münster, Germany and 3 Abteilung für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum, Albert-Einstein-Allee 11, Ulm, Germany

Received on April 20, 2004; revised on June 1, 2004; accepted on June 16, 2004

Capsular hyaluronan of Streptococcus pyogenes is synthesized at the protoplast membrane. It is widely assumed that hyaluronan is exported by the synthase itself and that no additional protein is required for transfer through plasma membranes. However, we produced an insertional mutation that reduced the mucoid phenotype, hyaluronan production, and capsule formation. Nucleotide sequence analysis of the insertion site identified a gene coding for a protein with an ATP-binding cassette (ABC) that belonged to an ABC transporter system and was located next to the hyaluronan synthesis genes. The mucoid phenotype was reconstituted by complementation with DNA encoding the ABC transporter system. These results indicated that an ABC transporter was required for efficient capsule production.

1 To whom correspondence should be addressed; e-mail: prehm{at}uni-muenster.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. H. Weigel and P. L. DeAngelis
Hyaluronan Synthases: A Decade-plus of Novel Glycosyltransferases
J. Biol. Chem., December 21, 2007; 282(51): 36777 - 36781.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Schulz, U. Schumacher, and P. Prehm
Hyaluronan Export by the ABC Transporter MRP5 and Its Modulation by Intracellular cGMP
J. Biol. Chem., July 20, 2007; 282(29): 20999 - 21004.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kumari, B. A. Baggenstoss, A. L. Parker, and P. H. Weigel
Mutation of Two Intramembrane Polar Residues Conserved within the Hyaluronan Synthase Family Alters Hyaluronan Product Size
J. Biol. Chem., April 28, 2006; 281(17): 11755 - 11760.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Misra, S. Ghatak, and B. P. Toole
Regulation of MDR1 Expression and Drug Resistance by a Positive Feedback Loop Involving Hyaluronan, Phosphoinositide 3-Kinase, and ErbB2
J. Biol. Chem., May 27, 2005; 280(21): 20310 - 20315.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. Kumari and P. H. Weigel
Identification of a membrane-localized cysteine cluster near the substrate-binding sites of the Streptococcus equisimilis hyaluronan synthase
Glycobiology, May 1, 2005; 15(5): 529 - 539.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.