Glycobiology Advance Access originally published online on December 17, 2002
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Glycobiology, 2003, Vol. 13, No. 6 427-434
© 2003 Oxford University Press
Immunoreactivity in mammals of two typical plant glyco-epitopes, core 
(1,3)-fucose and core xylose
3 CNRS-UMR 6037, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan Cédex, France
4 INSERM U547, Institut Pasteur de Lille, 59019 Lille, France
5 INSERM U519, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan Cédex, France
6 INSERM U413, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan Cédex, France
Received on August 27, 2002; revised on October 22, 2002; accepted on October 24, 2002
The presence of nonmammalian core 
(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (which are the crops intended for the production of therapeutic proteins) and by reinvestigating their immunogenicity in rodents. We found that immunization with a model glycoprotein, horseradish peroxidase, elicits in C57BL/6 mice and rats the production of antibodies (Abs) specific for core (1,3)-fucose and core xylose epitopes. Furthermore, we demonstrated that about 50% of nonallergic blood donors contains in their sera Abs specific for core xylose, whereas 25% have Abs against core (1,3)-fucose. These Abs probably result from sensitization to environmental antigens. Although the immunological significance of these data is too speculative at the moment, the presence of such Abs might introduce some limitations to the use of plant-derived biopharmaceutical glycoproteins, such as an accelerated clearance during human therapy.
Present address: Glycobiology Research and Training Center, Department of Medicine and Cellular Medicine, University of California, San Diego, La Jolla, CA 92093-0687, USA
2 To whom correspondence should be addressed; e-mail: plerouge{at}crihan.fr
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