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Glycobiology Advance Access originally published online on December 17, 2002
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Glycobiology, 2003, Vol. 13, No. 4 265-277
© 2003 Oxford University Press

The contribution of tandem repeat number to the O-glycosylation of mucins

Howard Scott Silverman2, Mark Sutton-Smith3, Kimberley McDermott4, Paul Heal2, Shih-Hsing Leir2, Howard R. Morris3, Michael A. Hollingsworth4, Anne Dell3 and Ann Harris1,2

2 Paediatric Molecular Genetics, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK
3 Department of Biological Sciences, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, UK
4 Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198–6805, USA

Received on August 5, 2002; revised on November 2, 2002; accepted on November 6, 2002

The serine- and threonine-rich tandem repeat (TR) units that make up the characteristic feature of mucin glycoproteins are often polymorphic with substantial genetic variation in TR number. The precise effect of TR number on O-glycosylation is not fully understood, although the TR number of several mucins may be associated with apparent susceptibility to certain human diseases. To evaluate the contribution of TR number to O-glycosylation, we generated a series of chimeric mucins carrying increasing numbers of TR units from the MUC5B mucin in the context of an epitope-tagged MUC1 mucin backbone. These mucins were expressed in Caco2 colon carcinoma cell clones and purified by immunoprecipitation. O-Glycosylation was investigated by western blotting with antibodies to known carbohydrate structures and by fast atom bombardment-mass spectrometry. Additional carbohydrate epitopes were detected with antibodies on chimeric mucins with a higher TR number in comparison to those with fewer TRs. Using mass spectrometry, higher-molecular-weight glycans were detected more frequently on the mucins with extended TRs compared to those with fewer TRs. However no novel carbohydrate structures were seen, suggesting that TR number does not affect the specificity of O-glycosylation.

1 To whom correspondence should be addressed; e-mail: aharris{at}molbiol.ox.ac.uk


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