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Glycobiology Advance Access originally published online on October 30, 2002
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Glycobiology, 2003, Vol. 13, No. 2 129-137
© 2003 Oxford University Press

A novel GlcNAc{alpha}1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica

Manfred Wuhrer2, Christiane Grimm2, Ulrich Zähringer3, Roger D. Dennis2, Clemens M. Berkefeld2, Mohamed A. Idris4 and Rudolf Geyer12

2 Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany
3 Division of Immunochemistry, Research Center Borstel, Center for Medicine and Biosciences, D-23845 Borstel, Germany
4 Department of Microbiology and Immunology, College of Medicine, Sultan Qaboos University, Muscat, Sultanate of Oman

Received on July 2, 2002; revised on August 29, 2002; accepted on August 30, 2002

The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAc{alpha}1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal– and human–F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAc{alpha}1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.

1 To whom correspondence should be addressed; e-mail: rudolf.geyer{at}biochemie.med.uni-giessen.de


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 GlycobiologyHome page
M. Wuhrer, C. Grimm, R. D. Dennis, M. A. Idris, and R. Geyer
The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal({beta}1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity
Glycobiology, February 1, 2004; 14(2): 115 - 126.
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