Glycobiology Advance Access originally published online on June 10, 2003
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Glycobiology, 2003, Vol. 13, No. 12 919-928
© 2003 Oxford University Press
Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene
2 INSERM U419, Institut de Biologie, 9 Quai Moncousu, 44093, Nantes Cedex, France; 3 Laboratoire d'Immunogénétique Moléculaire, Université Paul Sabatier, Faculté de Médecine, Bâtiment A2, 133 Route de Narbonne, 31062, Toulouse, Cedex 4, France; 4 Laboratoire d'Immunohématologie, Etablissement Français du Sang Pyrénées-Méditerranée, Avenue de Grande Bretagne, BP 3210, 31027, Toulouse, Cedex 3, France; and 5 Université Libre de Bruxelles, IBMM, B-6041 Gosselies, Belgium
Received on April 11, 2003; revised on May 23, 2003; accepted on May 23, 2003
A genomic DNA fragment corresponding to exon 7 of the human ABO gene was amplified from rats of several inbred and outbred strains. Five different sequences were obtained, four of them corresponding to A-type sequences and one to a B-type sequence based on the amino acids equivalent to residues at positions 266 and 268 of the human enzymes. In rats from inbred strains, a single A-type sequence and the unique B-type sequence were found, whereas some animals of outbred strains presented two or three A-type sequences along with the B-type sequence. The complete coding sequence of the B-type gene was obtained; identification of the exon–intron boundaries, determined by comparison with rat genomic sequences from data banks, revealed that the rat B-type gene structure is identical with that of the mouse Abo gene. Compared with the human ABO gene and the rat A gene, it lacks exon 4. Like the rat A gene (symbol: Abo), the rat B gene (symbol: Abo2) is located on chromosome 3q11–q12. It could be shown by transfection experiments that the B-type cDNA encodes an active B transferase. A transcript of the B gene was found ubiquitously, whereas the B antigen was only detected in a restricted set of tissues. These data indicate that rats have at least two distinct Abo genes, one monomorphic gene encoding a B-specific enzyme and one or more genes in some cases encoding an A-specific enzyme.
1 To whom correspondence should be addressed; email: jlependu{at}nantes.inserm.fr
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