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Glycobiology, 2001, Vol. 11, No. 3 209-216
© 2001 Oxford University Press

Human {alpha}3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNAcß1-4(Fuc{alpha}1-3)GlcNAcß1-4R determinant at the nonreducing terminus

Jari Natunen, Olli Aitio, Jari Helin, Hannu Maaheimo1, Ritva Niemelä, Sami Heikkinen and Ossi Renkonen2

Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Finland

Human {alpha}3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Galß1-4(Fuc{alpha}1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAcß1-4GlcNAc, generating GalNAcß1-4(Fuc{alpha}1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2–4 GlcNAc units efficiently to products containing a GlcNAcß1-4(Fuc{alpha}1-3)GlcNAcß1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.

1 Present address: VTT Biotechnology, P.O. Box 1500, 02044 VTT, Finland.

2 To whom correspondence should be addressed


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H. Boer, N. Munck, J. Natunen, G. Wohlfahrt, H. Soderlund, O. Renkonen, and A. Koivula
Differential recognition of animal type {beta}4-galactosylated and {alpha}3-fucosylated chito-oligosaccharides by two family 18 chitinases from Trichoderma harzianum
Glycobiology, December 1, 2004; 14(12): 1303 - 1313.
[Abstract] [Full Text] [PDF]


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