Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gowda, D. C.
Right arrow Articles by Vogel, C.-W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gowda, D. C.
Right arrow Articles by Vogel, C.-W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, 2001, Vol. 11, No. 3 195-208
© 2001 Oxford University Press

N-linked oligosaccharides of cobra venom factor contain novel {alpha}(1-3)galactosylated Lex structures

D. Channe Gowda1,5, John Glushka6, Herman van Halbeek2,6, Rao N. Thotakura7, Reinhard Bredehorst3,5 and Carl-Wilhelm Vogel4,5

5Department of Biochemistry and Molecular Biology, and Vincent T. Lombardi Cancer Center, Georgetown University Medical Center, 3900 Reservoir Road, NW, Washington, DC 20007, USA, 6Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA, and 7Molecular and Cellular Endocrinology Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA

Cobra venom factor (CVF), a nontoxic, complement-activating glycoprotein in cobra venom, is a functional analog of mammalian complement component C3b. The carbohydrate moiety of CVF consists exclusively of N-linked oligosaccharides with terminal {alpha}1-3-linked galactosyl residues, which are antigenic in human. CVF has potential for several medical applications, including targeted cell killing and complement depletion. Here, we report a detailed structural analysis of the oligosaccharides of CVF. The structures of the oligosaccharides were determined by lectin affinity chromatography, antibody affinity blotting, compositional and methylation analyses, and high-resolution 1H-NMR spectroscopy. Approximately 80% of the oligosaccharides are diantennary complex-type, ~12% are tri- and tetra-antennary complex-type, and ~8% are oligomannose type structures. The majority of the complex-type oligosaccharides terminate in Gal{alpha}1-3Galß1-4(Fuc{alpha}1-3)GlcNAcß1, a unique carbohydrate structural feature abundantly present in the glycoproteins of cobra venom.

1 To whom correspondence should be addressed

2 Present address: Glycobiology Research and Training Center, School of Medicine, University of California at San Diego, La Jolla, CA 92093-0687, USA.

3 Present address: Department of Biochemistry and Molecular Biology, University of Hamburg, Hamburg 20146, Germany.

4 Present address: Cancer Research Center of Hawaii, University of Hawaii, 1236 Lauhala Street, Honolulu, HI 96813, USA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
K. Moriguchi, T. Takemoto, T. Aoki, S.-i. Nakakita, S. Natsuka, and S. Hase
Free Oligosaccharides with Lewis x Structure Expressed in the Segmentation Period of Zebrafish Embryo
J. Biochem., August 1, 2007; 142(2): 213 - 227.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Kock, B. E. Hew, H. Bammert, D. C. Fritzinger, and C.-W. Vogel
Structure and Function of Recombinant Cobra Venom Factor
J. Biol. Chem., July 16, 2004; 279(29): 30836 - 30843.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.