Glycobiology, 2001, Vol. 11, No. 11 969-977
© 2001 Oxford University Press
Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin
2Wellcome Trust Centre for Cell-Matrix Research, Division of Biochemistry, School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Manchester, M13 9PT, UK; 3Mucosal Biology Group, Department of Cell and Molecular Biology, Lund University, BMC, C-13, SE-22184, Lund, Sweden; 4St. George’s Hospital Medical School, Department of Physiology, Cranmer Terrace, London, SW17 ORE, UK.
Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28–1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS–PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average Mr of 5 x 105 with a range of Mr from 2 x 105 to 1 x 106. Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.
1 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. H. Kim, S. H. Lee, H. M. Lee, H. H. Jung, S. H. Lee, W. S. Cho, Y. G. Cinn, H. Choe, M. P. Kim, I. O. Yoo, et al. Increased Expression of Glycoprotein 340 in the Ethmoid Sinus Mucosa of Patients With Chronic Sinusitis Arch Otolaryngol Head Neck Surg, November 1, 2007; 133(11): 1111 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Thornton and J. K. Sheehan From Mucins to Mucus: Toward a More Coherent Understanding of This Essential Barrier Proceedings of the ATS, January 1, 2004; 1(1): 54 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Hartshorn, M. R. White, T. Mogues, T. Ligtenberg, E. Crouch, and U. Holmskov Lung and salivary scavenger receptor glycoprotein-340 contribute to the host defense against influenza A viruses Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1066 - L1076. [Abstract] [Full Text] [PDF]
|
|