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Glycobiology, 2001, Vol. 11, No. 1 47-55
© 2001 Oxford University Press

Structure of O-glycosidically linked oligosaccharides from glycoproteins of Trypanosoma cruzi CL-Brener strain: evidence for the presence of O-linked sialyl-oligosaccharides

Adriane R. Todeschini, Erika Xavier da Silveira, Christopher Jones3, Robin Wait1,4, José O. Previato and Lúcia Mendonça-Previato2

Instituto de Microbiologia, CCS-Bloco I, Universidade Federal do Rio de Janeiro, 21944–970-Cidade Universitária, Rio de Janeiro-RJ, Brasil, 3Laboratory for Molecular Structure, NIBSC, Blanche Lane, South Mimms, Herts, EN6 3QG, UK, and 4Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, SP4 0JG, UK

Glycoproteins on the cell surface of Trypanosoma cruzi are known to play important roles in the interaction of the parasite with the host cells. We previously determined the structures of the O-glycan chains from the sialoglycoproteins (mucin-like molecules) of the G- and Y-strains and observed significant differences between them. We now report the structures of the sialylated and nonsialylated O-linked oligosaccharides isolated from the cell surface glycoproteins of the myotropic CL-Brener strain grown in the presence of fetal calf serum. The structures of the O-linked oligosaccharide alditols obtained by reductive ß-elimination of the sialoglycoprotein were determined by a combination of methylation analysis, fast atom bombardment–mass spectrometry and nuclear magnetic resonance spectroscopy. The presence of a ß-galactopyranose substituent on the N-acetylglucosamine O-4 position shows that these O-linked oligosaccharides from CL-Brener strain belong to the same family as those isolated from mucins expressed by T. cruzi Y strain, a reticulotropic strain. In addition, novel O-glycans, including {alpha}2–3 mono-sialylated species are described.

1 Present address: Kennedy Institute for Rheumatology, 1 Aspenlea Road, London W6 8LH, UK

2 To whom correspondence should be addressed 3This paper is dedicated to the memory of Prof. Andre Verbert, our friend.


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