Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Uchibori-Iwaki, H.
Right arrow Articles by Ogawa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uchibori-Iwaki, H.
Right arrow Articles by Ogawa, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, 2000, Vol. 10, No. 9 865-874
© 2000 Oxford University Press

The changes in glycosylation after partial hepatectomy enhance collagen binding of vitronectin in plasma

Haruhi Uchibori-Iwaki3, Atsuko Yoneda1,3, Sachie Oda-Tamai4, Shigemi Kato4, Nobu Akamatsu4, Megumi Otsuka5, Kotono Murase6, Kyoko Kojima6, Risa Suzuki6, Yuko Maeya3, Mayumi Tanabe3 and Haruko Ogawa2,3

3Course of Advanced Biosciences, Graduate School of Humanities and Sciences, 4Department of Biochemistry, St. Marianna University School of Medicine, Sugao, Miayamae-ku, Kawasaki, Kanagawa, Japan, 5Department of Nutrition and Food Science and 6Department of Chemistry, Faculty of Sciences, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo 112–8610, Japan

Vitronectin is a multifunctional glycoprotein present in the extracellular matrix and plasma. Changes in rat vitronectin were studied during liver regeneration after partial hepatectomy. Carbohydrate concentrations of vitronectin decreased to 2/3 of sham-operated rats at 24 h after partial hepatectomy. Carbohydrate composition and lectin reactivity indicated that N-glycosylation and sialylation of vitronectin changed markedly after partial hepatectomy, while amino acid composition did not change significantly. We previously showed that deN-glycosylation of vitronectin in vitro affects collagen binding among various ligands (Yoneda et al., Biochemistry (1998) 37, 6351–6360). Vitronectins from partially hepatectomized rats at 24 h were found to exhibit markedly enhanced binding to type I collagen. The effect of sialylation on collagen binding was further examined using enzymatically deglycosylated vitronectin of nonoperated rats. Collagen binding increased by 1.2 times after deN-glycosylation of vitronectin, while it increased more than 2.9 times after desialylation. Various glycosyltransferases in liver are known to change after partial hepatectomy, including the attenuation of N-oligosaccharide transferase. The findings therefore suggest that the collagen binding of vitronectin is modulated by the alteration of peptide glycosylation caused by postoperative physiological changes of glycosyltransferases and that the change may contribute to tissue remodeling processes.

1 Present address: Biosignaling Department, National Institute of Bioscience and Human Technology, 1–1 Higashi, Tsukuba, Ibaraki, Japan

2 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
K. Sano, K. Asanuma-Date, F. Arisaka, S. Hattori, and H. Ogawa
Changes in glycosylation of vitronectin modulate multimerization and collagen binding during liver regeneration
Glycobiology, July 1, 2007; 17(7): 784 - 794.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
Z. Yasukawa, C. Sato, K. Sano, H. Ogawa, and K. Kitajima
Identification of disialic acid-containing glycoproteins in mouse serum: a novel modification of immunoglobulin light chains, vitronectin, and plasminogen
Glycobiology, July 1, 2006; 16(7): 651 - 665.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Takekawa, C. Ina, R. Sato, K. Toma, and H. Ogawa
Novel Carbohydrate-binding Activity of Pancreatic Trypsins to N-Linked Glycans of Glycoproteins
J. Biol. Chem., March 31, 2006; 281(13): 8528 - 8538.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Matsushita, M. Takenaka, and H. Ogawa
Porcine Pancreatic alpha -Amylase Shows Binding Activity toward N-Linked Oligosaccharides of Glycoproteins
J. Biol. Chem., February 8, 2002; 277(7): 4680 - 4686.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.