Glycobiology, 2000, Vol. 10, No. 4 431-438
© 2000 Oxford University Press
Siglec-7: a sialic acid-binding lectin of the immunoglobulin superfamily
Glycobiology Research and Training Center, Department of Medicine and Cancer Center, University of California San Diego, La Jolla, CA 92093, USA
The Siglecs are a recently discovered family of sialic acid-binding lectins of the immunoglobulin (Ig) superfamily. We report a molecule showing homology to the six first reported Siglecs, with the closest relationship to Siglec-3(CD33), Siglec-5, and Siglec-6(OBBP-1). The extracellular portion has two Ig-like domains, with the amino-terminal V-set Ig domain including amino acid residues known to be involved in sialic acid recognition by other Siglecs. The cytoplasmic domain has putative sites of tyrosine phosphorylation shared with some Siglecs, including an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). Expression of the full-length cDNA induces sialic acid-dependent binding to human erythrocytes. A recombinant chimeric form containing the extracellular Ig domains selectively recognizes the sequence Neu5Ac
2–6Galß1–4Glc, and binding requires the side chain of sialic acid. Mutation of an arginine residue predicted to be critical for sialic acid binding abolishes both interactions. Taken together, our findings justify designation of the molecule as Siglec-7. Analysis of bacterial artificial chromosome (BAC) clones spanning the known human genomic location of Siglec-3 indicates that the Siglec-7 gene is also located on chromosome 19q13.3–13.4. Human tissues show strong expression of Siglec-7 mRNA in spleen, peripheral blood leukocytes, and liver. The combination of an extracellular sialic acid binding site and an intracellular ITIM motif suggests that this molecule is involved in trans-membrane regulatory signaling reactions.
1 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Angata, Y. Tabuchi, K. Nakamura, and M. Nakamura Siglec-15: an immune system Siglec conserved throughout vertebrate evolution Glycobiology, August 1, 2007; 17(8): 838 - 846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Orr, N. M. Morgan, J. Elliott, J. F. Burrows, C. J. Scott, D. W. McVicar, and J. A. Johnston CD33 responses are blocked by SOCS3 through accelerated proteasomal-mediated turnover Blood, February 1, 2007; 109(3): 1061 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Attrill, A. Imamura, R. S. Sharma, M. Kiso, P. R. Crocker, and D. M. F. van Aalten Siglec-7 Undergoes a Major Conformational Change When Complexed with the {alpha}(2,8)-Disialylganglioside GT1b J. Biol. Chem., October 27, 2006; 281(43): 32774 - 32783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Angata, T. Hayakawa, M. Yamanaka, A. Varki, and M. Nakamura Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates FASEB J, October 1, 2006; 20(12): 1964 - 1973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Varki and T. Angata Siglecs--the major subfamily of I-type lectins Glycobiology, January 1, 2006; 16(1): 1R - 27R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tsuchida, M. Ogiso, Y. Nakamura, M. Kiso, K. Furukawa, and K. Furukawa Molecular Cloning and Expression of Human ST6GalNAc III: Restricted Tissue Distribution and Substrate Specificity J. Biochem., September 1, 2005; 138(3): 237 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ikehara, S. K. Ikehara, and J. C. Paulson Negative Regulation of T Cell Receptor Signaling by Siglec-7 (p70/AIRM) and Siglec-9 J. Biol. Chem., October 8, 2004; 279(41): 43117 - 43125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Miyazaki, K. Ohmori, M. Izawa, T. Koike, K. Kumamoto, K. Furukawa, T. Ando, M. Kiso, T. Yamaji, Y. Hashimoto, et al. Loss of Disialyl Lewisa, the Ligand for Lymphocyte Inhibitory Receptor Sialic Acid-Binding Immunoglobulin-Like Lectin-7 (Siglec-7) Associated with Increased Sialyl Lewisa Expression on Human Colon Cancers Cancer Res., July 1, 2004; 64(13): 4498 - 4505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Blixt, B. E. Collins, I. M. van den Nieuwenhof, P. R. Crocker, and J. C. Paulson Sialoside Specificity of the Siglec Family Assessed Using Novel Multivalent Probes: IDENTIFICATION OF POTENT INHIBITORS OF MYELIN-ASSOCIATED GLYCOPROTEIN J. Biol. Chem., August 15, 2003; 278(33): 31007 - 31019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. M. Brinkman-Van der Linden, T. Angata, S. A. Reynolds, L. D. Powell, S. M. Hedrick, and A. Varki CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice Mol. Cell. Biol., June 15, 2003; 23(12): 4199 - 4206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Collins, O. Blixt, N. V. Bovin, C.-P. Danzer, D. Chui, J. D. Marth, L. Nitschke, and J. C. Paulson Constitutively unmasked CD22 on B cells of ST6Gal I knockout mice: novel sialoside probe for murine CD22 Glycobiology, September 1, 2002; 12(9): 563 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Angata, S. C. Kerr, D. R. Greaves, N. M. Varki, P. R. Crocker, and A. Varki Cloning and Characterization of Human Siglec-11. A RECENTLY EVOLVED SIGNALING MOLECULE THAT CAN INTERACT WITH SHP-1 AND SHP-2 AND IS EXPRESSED BY TISSUE MACROPHAGES, INCLUDING BRAIN MICROGLIA J. Biol. Chem., June 28, 2002; 277(27): 24466 - 24474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Grobe and L. D. Powell Role of protein kinase C in the phosphorylation of CD33 (Siglec-3) and its effect on lectin activity Blood, May 1, 2002; 99(9): 3188 - 3196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamaji, T. Teranishi, M. S. Alphey, P. R. Crocker, and Y. Hashimoto A Small Region of the Natural Killer Cell Receptor, Siglec-7, Is Responsible for Its Preferred Binding to alpha 2,8-Disialyl and Branched alpha 2,6-Sialyl Residues. A COMPARISON WITH Siglec-9 J. Biol. Chem., February 15, 2002; 277(8): 6324 - 6332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Angata, N. M. Varki, and A. Varki A Second Uniquely Human Mutation Affecting Sialic Acid Biology J. Biol. Chem., October 19, 2001; 276(43): 40282 - 40287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Matsui, L. Borsig, S. D. Rosen, M. Yaghmai, A. Varki, and S. H. Embury P-selectin mediates the adhesion of sickle erythrocytes to the endothelium Blood, September 15, 2001; 98(6): 1955 - 1962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Angata and A. Varki Cloning, Characterization, and Phylogenetic Analysis of Siglec-9, a New Member of the CD33-related Group of Siglecs. EVIDENCE FOR CO-EVOLUTION WITH SIALIC ACID SYNTHESIS PATHWAYS J. Biol. Chem., July 14, 2000; 275(29): 22127 - 22135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vinson, P. J. L. M. Strijbos, A. Rowles, L. Facci, S. E. Moore, D. L. Simmons, and F. S. Walsh Myelin-associated Glycoprotein Interacts with Ganglioside GT1b. A MECHANISM FOR NEURITE OUTGROWTH INHIBITION J. Biol. Chem., June 1, 2001; 276(23): 20280 - 20285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Li, W. Zhang, T. Wan, J. Zhang, T. Chen, Y. Yu, J. Wang, and X. Cao Cloning and Characterization of Siglec-10, a Novel Sialic Acid Binding Member of the Ig Superfamily, from Human Dendritic Cells J. Biol. Chem., July 20, 2001; 276(30): 28106 - 28112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yu, C.-M. Lai, M. Maoui, D. Banville, and S.-H. Shen Identification and Characterization of S2V, a Novel Putative Siglec That Contains Two V Set Ig-like Domains and Recruits Protein-tyrosine Phosphatases SHPs J. Biol. Chem., June 22, 2001; 276(26): 23816 - 23824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Angata, R. Hingorani, N. M. Varki, and A. Varki Cloning and Characterization of a Novel Mouse Siglec, mSiglec-F. DIFFERENTIAL EVOLUTION OF THE MOUSE AND HUMAN (CD33) Siglec-3-RELATED GENE CLUSTERS J. Biol. Chem., November 21, 2001; 276(48): 45128 - 45136. [Abstract] [Full Text] [PDF]
|
|