Glycobiology, 2000, Vol. 10, No. 12 1271-1275
© 2000 Oxford University Press
Phosphatidylethanolamine is the donor of the phosphorylethanolamine linked to the 
1,4-linked mannose of yeast GPI structures
Institute of Biochemistry, University of Fribourg, CH-1700 Fribourg, Switzerland
Glycosylphosphatidylinositol (GPI) anchors of all species contain the core structure protein-CO-NH-(CH2)2-PO4-Man
1–2Man1–6Man1–4GlcN1–6inositol-PO4-lipid. In recent studies in yeast it was found that gpi10-1 mutants accumulate M2, an abnormal intermediate having the structure Man1–6[NH2-(CH2)2-PO4
]Man1–4GlcN1–6(acyl)inositol-PO4-lipid. It thus was realized that yeast GPI lipids, as their mammalian counterparts, contain an additional phosphorylethanolamine side chain on the 1,4-linked mannose. The biosynthetic origin of this phosphorylethanolamine group was investigated using gpi10-1 
ept1 cpt1, a strain which is unable to synthesize phosphatidylethanolamine by transferring phosphorylethanolamine from CDP-ethanolamine onto diacylglycerol, but which still can make phosphatidylethanolamine by decarboxylation of phosphatidylserine. Gpi10-1 ept1 cpt1 triple mutants are unable to incorporate [3H]ethanolamine into M2 although metabolic labeling with [3H]inositol demonstrates that they make as much M2 as gpi10-1. In contrast, when labeled with [3H]serine, the triple mutant incorporates more label into M2 than gpi10-1. This result establishes that the phosphorylethanolamine group on the 1,4-linked mannose is derived from phosphatidylethanolamine and not from CDP-ethanolamine.
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