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Glycobiology, 2000, Vol. 10, No. 1 89-101
© 2000 Oxford University Press

Schistosoma mansoni cercarial glycolipids are dominated by Lewis X and pseudo-Lewis Y structures

Manfred Wuhrer, Roger D. Dennis, Michael J. Doenhoff2, Günter Lochnit and Rudolf Geyer1

Institute of Biochemistry, University of Giessen, D-35392 Giessen, Germany, and 2School of Biological Sciences, University of Wales, Bangor, Wales LL57 2UW, UK

The oligosaccharide structures of glycolipids from cercariae of the human blood fluke, Schistosoma mansoni, were analyzed in the form of their corresponding, pyridylaminated oligosaccharides by methylation analysis, partial hydrolysis, exoglycosidase treatment, on-target exoglyco­sidase cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The six, dominant chemical structures present have been determined as: GalNAc(ß1–4)Glc1-ceramide; GlcNAc(ß1–3)Gal­NAc(ß1–4)Glc1-ceramide; Gal(ß1–4)GlcNAc(ß1–3)Gal­NAc(ß1–4)Glc1-ceramide; Gal(ß1–4)[Fuc({alpha}1–3)]Glc­NAc(ß1–3)Gal­NAc(ß1–4)Glc1-ceramide (Lewis X pentasaccharide structure); Gal(ß1–4)[Fuc({alpha}1–3)]GlcNAc(ß1–3)Glc­NAc(ß1–3)GalNAc(ß1–4)Glc1-ceramide (Lewis X hexa­saccharide structure); and, Fuc({alpha}1–3)Gal(ß1–4)[Fuc({alpha}1–3)]GlcNAc(ß1–3)GalNAc(ß1–4)Glc1-ceramide (pseudo-Lewis Y hexasaccharide structure). These structures belong to the characterized schisto-series of protostomial glycosphingolipids. The Lewis X and pseudo-Lewis Y glyco­lipids are stage-specifically expressed by the cercarial life-cycle stage, and not by the adult or egg.

1 To whom correspondence should be addressed at: Biochemisches Institut am Klinikum der Universität, Friedrichstrasse 24, D-35392 Giessen, Germany


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