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Glycobiology vol 1 no 2 pp. 173-185, 1991
© 1991

research-article

A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator

Anna L. Chan, Howard R. Morris3, Maria Panico, A. Tony Etienne, Mark E. Rogers1, Patrick Gaffney2, Lesley Creighton-Kempsford2 and Anne Dell3

Departments of Biochemistry, Imperial College of Science Technology and Medicine London SW7 2AZ
1 M-Scan Ltd, Silwood Park, Ascot, Berks SL5 7PZ UK
2National Institute for Biological Standards and Control Potters Bar, Herts EN6 3QG, UK

3To whom correspondence should be addressed

Received on November 1, 1990; accepted on November 14, 1990

We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1->4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins.

fast atom bombardment mass spectrometry N-glycans novel structures sialylated GalNAc tissue plasminogen activator


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